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KMID : 0545120000100020264
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.264 ~ p.266
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Improved T-Vector for the Cloning of PCR DNA Using Green Fluorescent Protein
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PARK, KILL SOON
PARK, SEONG WEON/CHOI, SOON-YONG
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Abstract
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A new GFP-based T-vector for cloning of PCR products was developed by using a green fluorescent protein (GFP) as a marker. In order to facilitate the DNA inserts, multiple restriction sites, SP6 and T7 RNA polymerase promoter sites, were introduced close to the PCR DNA insertion site of a pCRGv vector. The XcmI-digested pHNT plasmid can be used to clone a 3´ A-overhanged PCR DNA amplified by Taq DNA polymerase. A potential method of easing some difficulties from its use along with its cost savings provided by this vector are likely to lead to the replacement of other T-vectors for PCR DNA cloning.
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